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Valve issues, fluidics leaking onto electronics, flow cell Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Accuri C6 is a digital PC based bench top analyser, 2 laser system. Standard fluorochrome in use are identical to FACS Calibur. Even more colors to choose from with selectable lasers and filter replacement. Available to trained users with online booking. Optional filters and the Selectable Lasers Module expand the available fluorochrome combinations. During manufacture, laser and optical alignments are set and locked down.

This portable and compact system holds the analytical power of a cytometer twice its size. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. This is a modal window. Beginning of dialog window. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Additional laser configurations (2-blue 2-red, 4-blue) are available with the Selectable Lasers Module (Cat. No. 661085).

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The maximum recommended sample volume in a 12 x 75-mm tube is 2 mL. Never acquire more than 750 μ L from a single tube using the Medium fluidics setting, or … Flow Cytometry within Reach The BD Accuri™ C6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. The analytical power and versatility of today’s laser-based flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. 2018-06-08 Laser: Ex (nm) Em (nm) Channel: Fluorochromes: Blue: 488: 530/30: FL-1: FITC, Alexa Fluor 488, GFP, YFP, CFSE, CFDA, Fluo-3, Fluo-4, Qdot 525, JC1, DCFH: Blue: 488 BD Accuri C6 (1) 2 lasers: Blue and Red Up to 4 color parameters Beckman Coulter Vi-CELL XR (1) Viability cell counter with PI Sorters: BD FACS Aria II SORP (1) 4 solid-state lasers: Violet, Blue, Green, Red Up to 14 color parameters I met the same issue.

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This flow cytometer is rugged and designed to withstand harsh The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you BD Accuri™ C6 BD FACSCalibur™ BD FACSVerse™* BD FACSCanto™ II BD LSRFortessa™ X-20 BD FACSAria™ Product Family BD Influx™ BD FACSJazz™ Excitation Laser Line Filter Relative Brightness Blue Laser (488 nm) / Yellow-Green Laser (561 nm) •••••••• 488 nm … BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE..1 1.1 Starting BD Accuri C6 Software APPENDIX E SELECTABLE LASERS..86 E.1 Installing the Selectable Lasers Module This small but powerful Accuri C6 comes computer loaded with BD Accuri™ C6 Plus software. The BD Accuri™ C6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. It is a personal flow cytometer that brings cell analysis within reach by easy to use, simple to maintain, and affordable. Lasers Module (Cat. No. 653126) allows reassignment of the standard laser-detector associations, and optional filters can modify the effective detector characteristics. In fact, researchers have found that the compact, affordable BD Accuri C6 can replace multiple specialized flow cytometry systems. Quantification of microorganisms No. Our machine is still sitting in San Jose at BD over a year later because we haven't decided if it's worth paying for the repairs.

Facilitates standard three detectors off blue laser, and one off red laser. Allows for four blue detectors, or two blue and two red detectors. Regulatory Status RUO BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. 1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3.
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Standard fluorochrome in use are identical to FACS Calibur. Even more colors to choose from with selectable lasers and filter replacement. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood.
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The detector ranges are found in the charts below. To design a panel using Fluorofinder please click here. BLUE 488 LASER (10mW): Measures two scatter parameters: FSC and SSC. Also measures the following stains: Accuri C6 Software automatically displays the percentage of cells within the region. Gates cannot only be deleted from the workspace by deleting the plot on which it was drawn. Gate names are assigned sequentially by type (R1, R2 R3, P1, P2, P3, M1, M2 etc). as created.

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4 colors 2015-07-07 Our Accuri Gen 1 had to be replaced as BD can now longer service it. We had no redundancy in the lab for flow cytometry. Lori McDonald of Certified GeneTool Inc. found us a replacement Accri Gen 2 and provided QC bead tests after it’s last PM, and before shipping so that we could compare with our installation. She accomplished all this in less than a week and arranged for 2day delivery. The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515.

All current service contracts will be honored. Current extended warranties will not be affected and will be supported until they expire. 1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications. Laser Excitation.